Regulatory

Part:BBa_K177021:Design

Designed by: Kamil Koper   Group: iGEM09_Warsaw   (2009-07-08)


Promoter of the mgtC gene, operates with the PhoP/PhoQ system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 196
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Required the addition of DMSO and the use of a gradient PCR for successful amplification.


Source

Wild-type Salmonella typhimurium

References